Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells

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2.4K Views

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10:46 min

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February 2nd, 2022

DOI :

10.3791/63425-v

February 2nd, 2022

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Himanshu Pandey¹, Tatiana Zvagelsky¹, Mary Popov¹, Mayan Sadan¹, Neta Yanir¹, Alina Goldstein-Levitin¹, Nurit Siegler¹, Shira Hershfinkel¹, Yahel Abraham¹, Roy Avraham¹, Levi A. Gheber², Larisa Gheber¹

¹Department of Chemistry, Ben-Gurion University of the Negev, Israel, ²Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Israel

Podsumowanie

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Cin8

Kinesin 5

Bidirectional Motor Protein

Saccharomyces Cerevisiae

Single molecule Motility

Purification Protocol

GFP type Cin8

Microtubules

Protein Overexpression

Clusters

Fluorescent Particles

Galactose Induction

Nickel NTA Column

Yeast selective Medium

Rozdziały w tym wideo

0:05

Introduction

1:51

Cin8 Overexpression and Purification from Saccharomyces cerevisiae Cells

4:06

Single-Molecule Motility Assay with the Purified Cin8

6:29

Motility Analysis

9:13

Results: Motility Characteristics of Different Cluster Sizes of Cin8 on Single Microtubules

9:58

Conclusion

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