Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform (RpEGEN) for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium

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2.4K Views

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13:12 min

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March 2nd, 2022

DOI :

10.3791/63658-v

March 2nd, 2022

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Lyndsay L. Leach¹, G. Burch Fisher², Jeffrey M. Gross¹^,³

¹Department of Ophthalmology, Louis J. Fox Center for Vision Restoration, University of Pittsburgh School of Medicine, ²Earth Research Institute, University of California, Santa Barbara, ³Department of Developmental Biology, University of Pittsburgh School of Medicine

Podsumowanie

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Nitroreductase

Metronidazole

Retinal Pigment Epithelium

Pharmacological Treatment

Fluorescent Microscopy

Rozdziały w tym wideo

0:05

Introduction

1:00

Screening Zebrafish Larvae for rpe65a:nfsB-eGFP and Genetic Ablation of the Retinal Pigment Epithelium

3:02

Incorporating Pharmacological Treatment into Zebrafish Retinal Pigment Epithelium Ablation Protocol

6:35

Confocal Microscope Z-stack Image Preprocessing in FIJI (ImageJ)

8:19

Quantification and Visualization of RPE Regeneration Using RpEGEN Scripts

10:42

Results: RPE Expression, Ablation, Regeneration Quantification

12:39

Conclusion

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