Zaloguj się

Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform (RpEGEN) for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium

2.4K Views

13:12 min

March 2nd, 2022

DOI :

10.3791/63658-v

March 2nd, 2022


Przeglądaj więcej filmów

Nitroreductase

Rozdziały w tym wideo

0:05

Introduction

1:00

Screening Zebrafish Larvae for rpe65a:nfsB-eGFP and Genetic Ablation of the Retinal Pigment Epithelium

3:02

Incorporating Pharmacological Treatment into Zebrafish Retinal Pigment Epithelium Ablation Protocol

6:35

Confocal Microscope Z-stack Image Preprocessing in FIJI (ImageJ)

8:19

Quantification and Visualization of RPE Regeneration Using RpEGEN Scripts

10:42

Results: RPE Expression, Ablation, Regeneration Quantification

12:39

Conclusion

Powiązane Filmy

article

10:31

Multicolor Time-lapse Imaging of Transgenic Zebrafish: Visualizing Retinal Stem Cells Activated by Targeted Neuronal Cell Ablation

16.4K Views

article

06:56

A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt

11.7K Views

article

07:27

Laser Ablation of the Zebrafish Pronephros to Study Renal Epithelial Regeneration

15.2K Views

article

10:19

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

11.3K Views

article

08:53

Methylnitrosourea (MNU)-induced Retinal Degeneration and Regeneration in the Zebrafish: Histological and Functional Characteristics

11.4K Views

article

07:58

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury

8.8K Views

article

09:35

Primary Cell Cultures from the Mouse Retinal Pigment Epithelium

20.9K Views

article

07:59

Primary Culture of Porcine Retinal Pigment Epithelial Cells

2.1K Views

article

06:21

Isolation of Primary Mouse Retinal Pigmented Epithelium Cells

4.8K Views

article

07:59

Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis

2.5K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone