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Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

2.4K Views

09:09 min

August 17th, 2022

DOI :

10.3791/63889-v

August 17th, 2022


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Bottom up Methods

Rozdziały w tym wideo

0:05

Introduction

0:49

Designing Wavy NOA (Norland Optical Adhesive) Replica from Wavy PDMS (Polydimethylsiloxane) Patterns

1:49

Generation of a Supported Lipid Bilayer and Protein Incubation

3:22

Sample Fixation, Sample Dehydration, Critical Point Drying

4:43

Sample Mounting and Coating

5:54

Acquire the Images Using a Field-Emission SEM (FE-SEM) Microscope

6:56

Results: Analysis of the Self-Assembly of Septins at the Lipid Membrane

8:27

Conclusion

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