Generation of a Supported Lipid Bilayer and Protein Incubation
3:22
Sample Fixation, Sample Dehydration, Critical Point Drying
4:43
Sample Mounting and Coating
5:54
Acquire the Images Using a Field-Emission SEM (FE-SEM) Microscope
6:56
Results: Analysis of the Self-Assembly of Septins at the Lipid Membrane
8:27
Conclusion
필기록
Using this protocol, we visualized the organization of cytoskeletal filamentous proteins on pattern substrates displaying various curvatures. We can test the curvature sensitivity. The resolution of scanning electron microscope images is high enou
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
Septins are cytoskeletal proteins. They interact with lipid membranes and can sense but also generate membrane curvature at the micron scale. We describe in this protocol bottom-up in vitro methodologies for analyzing membrane deformations, curvature-sensitive septin binding, and septin filament ultrastructure.