Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

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2.4K Views

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10:09 min

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September 16th, 2022

DOI :

10.3791/64205-v

September 16th, 2022

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Daisuke Kato¹^,², Xiangyu Quan³, Yuta Tanisumi¹^,², Zhongtian Guo¹^,², Mitsuhiro Morita⁴, Tetsuya Takiguchi⁵, Osamu Matoba⁶, Hiroaki Wake¹^,²^,⁶

¹Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, ²Division of Multicellular Circuit Dynamics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, ³Department of System Science, Kobe University Graduate School of System Informatics, ⁴Department of Biology, Graduate School of Sciences, Kobe University, ⁵Department of Information Science, Kobe University Graduate School of System Informatics, ⁶Center of Optical Scattering Image Science, Kobe University

Podsumowanie

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Neural Activity

Two photon Holographic Microscopy

Neuroactivity Evaluation

Spatial Temporal Resolution

Neuropsychiatric Disorders

Craniotomy Procedure

AAV Solution Injection

Artificial Spinal Fluid

Brain Surface Cleaning

Glass Capillary Injection

Holographic Stimulation System

Live Imaging Mode

Calibration GUI

Dental Adhesive Resin Cement

Rozdziały w tym wideo

0:04

Introduction

0:55

Surgery and Adeno‐Associated Virus (AAV) Injection

2:50

Preparation for the Holographic Stimulation or Illumination System

4:54

Ca²⁺ Imaging Using an Image Sensor with Holographic Illumination

6:06

Two‐Photon Imaging with Optogenetics Using a Holographic Microscope

8:04

Results: Imaging and Functional Connectivity Using a Holographic Microscope

9:26

Conclusion

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