Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
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10:09 min
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September 16th, 2022
DOI :
September 16th, 2022
•Daisuke Kato1,2, Xiangyu Quan3, Yuta Tanisumi1,2, Zhongtian Guo1,2, Mitsuhiro Morita4, Tetsuya Takiguchi5, Osamu Matoba6, Hiroaki Wake1,2,6
1Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, 2Division of Multicellular Circuit Dynamics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 3Department of System Science, Kobe University Graduate School of System Informatics, 4Department of Biology, Graduate School of Sciences, Kobe University, 5Department of Information Science, Kobe University Graduate School of System Informatics, 6Center of Optical Scattering Image Science, Kobe University
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Chapters in this video
0:04
Introduction
0:55
Surgery and Adeno‐Associated Virus (AAV) Injection
2:50
Preparation for the Holographic Stimulation or Illumination System
4:54
Ca2+ Imaging Using an Image Sensor with Holographic Illumination
6:06
Two‐Photon Imaging with Optogenetics Using a Holographic Microscope
8:04
Results: Imaging and Functional Connectivity Using a Holographic Microscope
9:26
Conclusion
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