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Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

2.4K Views

06:37 min

December 23rd, 2022

DOI :

10.3791/64573-v

December 23rd, 2022


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Flotation based T Cell Isolation

Rozdziały w tym wideo

0:04

Introduction

0:29

Isolation of T Cells with Microbubbles Using Positive Selection

1:51

Co-Stimulation (Activation) of the Positively Selected T Cells

2:52

Expansion of the Co-Stimulated Cells in Cell Culture Medium

3:28

Expansion of the T Cells (With or Without Prior Transduction)

4:02

Harvesting the T Cells and Flow Cytometry

5:16

Results: Assessment of Viable and Successfully Transduced Cells

5:58

Conclusion

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