Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing
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1.7K Views
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05:34 min
•
February 10th, 2023
DOI :
February 10th, 2023
•Peibo Wang*1, Yongcan Zhou*1,3, Bei Wang2, Ahmed Elaswad4, Shifeng Wang1,3, Weiliang Guo1,3, Dongdong Zhang1,3
1State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, 2Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, College of Fishery, Guangdong Ocean University, 3Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science, Hainan University, 4Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University
Rozdziały w tym wideo
0:05
Introduction
0:42
Fish, Reagent, and Equipment Preparation
1:18
Tissue Dissection and Cell Dissociation
3:50
Staining and Microscopic Examination
4:16
Results: Effect of Enzymes on Tissue Dissociation
4:52
Conclusion
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