JoVE Journal

Neuroscience

This content is Free Access.

Focused Ultrasound Neuromodulation of Human In Vitro Neural Cultures in Multi-Well Microelectrode Arrays

Please note that all translations are AI generated. Click here for the English version.

1.2K Views

•

04:00 min

•

May 3rd, 2024

DOI :

10.3791/65115-v

May 3rd, 2024

•

Ruixing Liang¹^,², Griffin Mess²^,³, Joshua Punnoose²^,³, Kelley M. Kempski Leadingham²^,³, Constantin Smit²^,³, Nitish Thakor¹^,⁴, Christa W. Habela³, Betty Tyler³, Yousef Salimpour³, Amir Manbachi¹^,²^,³^,⁴^,⁵^,⁶

¹Electrical and Computer Engineering, Johns Hopkins University, ²HEPIUS Innovation Laboratory, Johns Hopkins University School of Medicine, ³Neurosurgery, Johns Hopkins University School of Medicine, ⁴Biomedical Engineering, Johns Hopkins University School of Medicine, ⁵Mechanical Engineering, Johns Hopkins University, ⁶Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine

Podsumowanie

Here, we present a protocol for using a high-throughput system that enables the monitoring and quantification of the neuromodulatory effects of focused ultrasound on human-induced pluripotent stem cell (HiPSC) neurons.

Przeglądaj więcej filmów

Focused Ultrasound

Neuromodulation

In Vitro Testing

Neural Cultures

Microelectrode Arrays

Stimulation Parameters

Neuronal Mechanism

Human Induced Pluripotent Stem Cells

Signal Analysis

Spikes Detection

Environmental Noise Filtering

Rozdziały w tym wideo

0:00

Introduction

0:37

Preparation of Materials and Setting Up the Experiment

1:23

Stimulation and Neuronal Signal Acquisition

2:18

Results: The Neuromodulatory Effects of Focused Ultrasound (FUS) on Neurons

3:07

Conclusion

Powiązane Filmy

article

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain

10.1K Views

article

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model

10.0K Views

article

Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays

9.7K Views

article

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays

14.5K Views

article

Stereotaxic Surgery for Implantation of Microelectrode Arrays in the Common Marmoset (Callithrix jacchus)

9.3K Views

article

Focused Ultrasound Induced Blood-Brain Barrier Opening for Targeting Brain Structures and Evaluating Chemogenetic Neuromodulation

3.4K Views

article

Construction and Implementation of Carbon Fiber Microelectrode Arrays for Chronic and Acute In Vivo Recordings

3.6K Views

article

Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays

2.7K Views

article

Pipeline for Planning and Execution of Transcranial Ultrasound Neuromodulation Experiments in Humans

841 Views

article

Simultaneous Focused Ultrasound Neuromodulation and Fiber Photometry Recording in Free-Moving Mouse

722 Views

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone

Używamy plików cookie, aby ulepszyć Twoje wrażenia z korzystania z naszej witryny.

Kontynuując korzystanie z naszej witryny lub klikając „Kontynuuj”, wyrażasz zgodę na akceptację naszych plików cookie.

Dowiedz się więcej