The research aims to provide a modified retina isolation workflow and tissue sample storage protocol, which is useful and practical for investigating RGCs. Retinas are fragile and folding, which limits experimental research when making a whole-mount retinal patch. In addition, all retinas do not require immediate immunostain.
Sometimes, extra retina samples need preservation for further use. We provide a detailed description of a reliable and technically straightforward method of whole-mount retina stain that emphasizes the proper preparation and the need for the long-term storage of retinas for RGC investigation. We will continue to work on the basic research of degenerative optic neuropathies.
Further, the molecular mechanism of optic nerve injury and accident regeneration and potential clinical targets for diagnosing and treating neurodegenerative disease will be explored.
