Embryo implantation, the initial step of a successful pregnancy is crucial for understanding its biological basis and addressing the challenges of infertility. The aim of this study is to establish a consistent platform for analyzing embryo endometrium interactions under controlled conditions. Based on this in vitro model we found that ultra high concentrations of estrogen significantly reduce the embryo implantation risk whereas progesterone supplementation could dependently alleviated these negative effects.
This study offers new insights and therapeutic strategies for improving pregnancy risks in fresh embryo transfer during controlled ovarian stimulation for IVF. Compared to three dimensional culture system this in vitro model offers a simple rapid and cost effective platform that can be established in most laboratories and exhibits high reproducibility and reliability making it an advantageous tool for research and development in the field of reproductive biology.
