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Single-Molecule FRET Imaging for Observing the Conformational Dynamics of Dynamin-Like GTPase Atlastin

335 Views

10:19 min

January 24th, 2025

DOI :

10.3791/67263-v

January 24th, 2025


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Biochemistry

Rozdziały w tym wideo

0:00

Introduction

1:22

Preparation and Modification of Coverslips for Protein Immobilization in Chambers

4:12

Protein Biotinylation and Fluorophore Labeling for Single-Molecule Frster Resonance Energy Transfer (smFRET) Assays

6:41

Single-Molecule FRET Imaging and Data Analysis for GTP Hydrolysis Cycle of Atlastin

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