Martin J. Lohse

New directions in pharmacology.

Internalization determinants of the parathyroid hormone receptor differentially regulate beta-arrestin/receptor association.

Inhibition of Na(+)-H(+) exchange prevents hypertrophy, fibrosis, and heart failure in beta(1)-adrenergic receptor transgenic mice.

Placental alpha(2)-adrenoceptors control vascular development at the interface between mother and embryo.

Magnesium and the parathyroid.

Modulation of a brain voltage-gated K+ channel by syntaxin 1A requires the physical interaction of Gbetagamma with the channel.

Dual regulation of the parathyroid hormone (PTH)/PTH-related peptide receptor signaling by protein kinase C and beta-arrestins.

Regulation of the inward rectifying properties of G-protein-activated inwardly rectifying K+ (GIRK) channels by Gbeta gamma subunits.

Feedback inhibition of catecholamine release by two different alpha2-adrenoceptor subtypes prevents progression of heart failure.

Mutation of Asn293 to Asp in transmembrane helix VI abolishes agonist-induced but not constitutive activity of the beta(2)-adrenergic receptor.

Regulation of phosducin-like protein by casein kinase 2 and N-terminal splicing.

The amino-terminal domain of G-protein-coupled receptor kinase 2 is a regulatory Gbeta gamma binding site.

Peptide G protein agonists from a phage display library.

Alterations in the myocardial creatine kinase system precede the development of contractile dysfunction in beta(1)-adrenergic receptor transgenic mice.

Differential control of adrenal and sympathetic catecholamine release by alpha 2-adrenoceptor subtypes.

Measurement of the millisecond activation switch of G protein-coupled receptors in living cells.

Partial agonist activity of bucindolol is dependent on the activation state of the human beta1-adrenergic receptor.

Disruption of cardiac Ena-VASP protein localization in intercalated disks causes dilated cardiomyopathy.

Direct optical recording of intrinsic efficacy at a G protein-coupled receptor.

What is the role of beta-adrenergic signaling in heart failure?

Common genomic response in different mouse models of beta-adrenergic-induced cardiomyopathy.

Protein kinase C switches the Raf kinase inhibitor from Raf-1 to GRK-2.

Gi protein activation in intact cells involves subunit rearrangement rather than dissociation.

Altered calcium handling is critically involved in the cardiotoxic effects of chronic beta-adrenergic stimulation.

Transgenic triadin 1 overexpression alters SR Ca2+ handling and leads to a blunted contractile response to beta-adrenergic agonists.

Calcium channel function and regulation in beta 1- and beta 2-adrenoceptor transgenic mice.

Beta-adrenoceptor polymorphisms and heart failure.

Molecular mechanisms of receptor activation: real-time analysis by fluorescence resonance energy transfer.

Direct evidence for a beta 1-adrenergic receptor-directed autoimmune attack as a cause of idiopathic dilated cardiomyopathy.

Fluorescence resonance energy transfer-based analysis of cAMP dynamics in live neonatal rat cardiac myocytes reveals distinct functions of compartmentalized phosphodiesterases.

Novel single chain cAMP sensors for receptor-induced signal propagation.

Kinetics of G-protein-coupled receptor signalling and desensitization.

Real-time monitoring of the PDE2 activity of live cells: hormone-stimulated cAMP hydrolysis is faster than hormone-stimulated cAMP synthesis.

Beta-arrestin binding to the beta2-adrenergic receptor requires both receptor phosphorylation and receptor activation.

Self-limitation of intravenous tocolysis with beta2-adrenergic agonists is mediated through receptor G protein uncoupling.

Phosducin-like protein regulates G-protein betagamma folding by interaction with tailless complex polypeptide-1alpha: dephosphorylation or splicing of PhLP turns the switch toward regulation of Gbetagamma folding.

A FlAsH-based FRET approach to determine G protein-coupled receptor activation in living cells.

G Protein activation without subunit dissociation depends on a G{alpha}(i)-specific region.

Interplay of Ca2+ and cAMP signaling in the insulin-secreting MIN6 beta-cell line.

The transcriptional repressor Nab1 is a specific regulator of pathological cardiac hypertrophy.

Peptide inhibitors of G protein-coupled receptor kinases.

Turn-on switch in parathyroid hormone receptor by a two-step parathyroid hormone binding mechanism.

Dynamics of receptor/G protein coupling in living cells.

Fluorescent sensors for rapid monitoring of intracellular cGMP.

beta(1)-Adrenergic receptor function, autoimmunity, and pathogenesis of dilated cardiomyopathy.

Molecular basis of inverse agonism in a G protein-coupled receptor.

SUMO-1 controls the protein stability and the biological function of phosducin.

Monitoring of cAMP synthesis and degradation in living cells.

Molecular basis of partial agonism at the neurotransmitter alpha2A-adrenergic receptor and Gi-protein heterotrimer.

Beta 1-adrenergic receptor-directed autoimmunity as a cause of dilated cardiomyopathy in rats.

Gs activation is time-limiting in initiating receptor-mediated signaling.

Cardiac myosin missense mutations cause dilated cardiomyopathy in mouse models and depress molecular motor function.

Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single cells.

Stimulating autoantibodies directed against the cardiac beta1-adrenergic receptor predict increased mortality in idiopathic cardiomyopathy.

Cyclic AMP imaging in adult cardiac myocytes reveals far-reaching beta1-adrenergic but locally confined beta2-adrenergic receptor-mediated signaling.

G protein-coupled receptors: too many dimers?

Interstitial remodeling in beta1-adrenergic receptor transgenic mice.

Real-time optical recording of beta1-adrenergic receptor activation reveals supersensitivity of the Arg389 variant to carvedilol.

Direct inhibition of cardiac hyperpolarization-activated cyclic nucleotide-gated pacemaker channels by clonidine.

cAMP microdomains and L-type Ca2+ channel regulation in guinea-pig ventricular myocytes.

A role for caspase-1 in heart failure.

International Union of Basic and Clinical Pharmacology. LXVII. Recommendations for the recognition and nomenclature of G protein-coupled receptor heteromultimers.

Requirements and ontology for a G protein-coupled receptor oligomerization knowledge base.

Live cell monitoring of mu-opioid receptor-mediated G-protein activation reveals strong biological activity of close morphine biosynthetic precursors.

A novel fluorescence method for the rapid detection of functional beta1-adrenergic receptor autoantibodies in heart failure.

Kinetic analysis of G protein-coupled receptor signaling using fluorescence resonance energy transfer in living cells.

Monitoring receptor signaling by intramolecular FRET.

Activation of AP-1 contributes to the beta-adrenoceptor-mediated myocardial induction of interleukin-6.

Conformational cross-talk between alpha2A-adrenergic and mu-opioid receptors controls cell signaling.

Kinetics of G-protein-coupled receptor signals in intact cells.

An RNA molecule that specifically inhibits G-protein-coupled receptor kinase 2 in vitro.

Optical techniques to analyze real-time activation and signaling of G-protein-coupled receptors.

Widespread receptivity to neuropeptide PDF throughout the neuronal circadian clock network of Drosophila revealed by real-time cyclic AMP imaging.

Real-time monitoring of phosphodiesterase inhibition in intact cells.

Integrin alpha E(CD103)beta 7 influences cellular shape and motility in a ligand-dependent fashion.

Cytoplasmic cAMP concentrations in intact cardiac myocytes.

Agonist-selective, receptor-specific interaction of human P2Y receptors with beta-arrestin-1 and -2.

Blocking them all: beta-arrestins inhibit cellular signaling.

Pathological autoantibodies in cardiomyopathy.

Dual role of the beta2-adrenergic receptor C terminus for the binding of beta-arrestin and receptor internalization.

A new type of ERK1/2 autophosphorylation causes cardiac hypertrophy.

Novel techniques for real-time monitoring of cGMP in living cells.

Critical role of transcription factor cyclic AMP response element modulator in beta1-adrenoceptor-mediated cardiac dysfunction.

Alterations of phospholamban function can exhibit cardiotoxic effects independent of excessive sarcoplasmic reticulum Ca2+-ATPase inhibition.

GPCR and G proteins: drug efficacy and activation in live cells.

Building a new conceptual framework for receptor heteromers.

Analysis of receptor oligomerization by FRAP microscopy.

Sweet taste receptor expressed in pancreatic beta-cells activates the calcium and cyclic AMP signaling systems and stimulates insulin secretion.

Cardiac hypertrophy: targeting Raf/MEK/ERK1/2-signaling.

A technique for monitoring multiple signals with a combination of prism-based total internal reflection fluorescence microscopy and epifluorescence microscopy.

Persistent cAMP-signals triggered by internalized G-protein-coupled receptors.

beta-Arrestin-2 interaction and internalization of the human P2Y1 receptor are dependent on C-terminal phosphorylation sites.

Gq-mediated Ca2+ signals inhibit adenylyl cyclases 5/6 in vascular smooth muscle cells.

Dimerization in GPCR mobility and signaling.

Phosducin influences sympathetic activity and prevents stress-induced hypertension in humans and mice.

Distinct pools of cAMP centre on different isoforms of adenylyl cyclase in pituitary-derived GH3B6 cells.

Agonist-regulated cleavage of the extracellular domain of parathyroid hormone receptor type 1.

A fluorescence resonance energy transfer-based M2 muscarinic receptor sensor reveals rapid kinetics of allosteric modulation.

Ca2+ cycling and new therapeutic approaches for heart failure.

Beta2-adrenergic receptor redistribution in heart failure changes cAMP compartmentation.

Signaling by internalized G-protein-coupled receptors.

Imaging of persistent cAMP signaling by internalized G protein-coupled receptors.

Targeting receptor antibodies in immune cardiomyopathy.

Polarization of migrating monocytic cells is independent of PI 3-kinase activity.

Site-specific, orthogonal labeling of proteins in intact cells with two small biarsenical fluorophores.

Cardiac beta1-adrenoceptor autoantibodies in human heart disease: rationale and design of the Etiology, Titre-Course, and Survival (ETiCS) Study.

GPCR-OKB: the G Protein Coupled Receptor Oligomer Knowledge Base.

Real-time monitoring of somatostatin receptor-cAMP signaling in live pituitary.

Microscopy: GPCR dimers moving closer.

Formation of a ternary complex among NHERF1, beta-arrestin, and parathyroid hormone receptor.

FRET-based sensors for the human M1-, M3-, and M5-acetylcholine receptors.

Sensing G protein-coupled receptor activation.

Differential signaling of the endogenous agonists at the beta2-adrenergic receptor.

Determination of onset of sexual maturation and mating behavior by melanocortin receptor 4 polymorphisms.

Fluorescent labeling of tetracysteine-tagged proteins in intact cells.

Rotational diffusion of the α(2a) adrenergic receptor revealed by FlAsH labeling in living cells.

Distinct pharmacological properties of morphine metabolites at G(i)-protein and β-arrestin signaling pathways activated by the human μ-opioid receptor.

FRET measurements of intracellular cAMP concentrations and cAMP analog permeability in intact cells.

Developing chemical genetic approaches to explore G protein-coupled receptor function: validation of the use of a receptor activated solely by synthetic ligand (RASSL).

Persistent cAMP signaling by internalized TSH receptors occurs in thyroid but not in HEK293 cells.

Nonequilibrium activation of a G-protein-coupled receptor.

Fluorescence/bioluminescence resonance energy transfer techniques to study G-protein-coupled receptor activation and signaling.

The guanine nucleotide exchange factor Vav2 is a negative regulator of parathyroid hormone receptor/Gq signaling.

Comparison of the activation kinetics of the M3 acetylcholine receptor and a constitutively active mutant receptor in living cells.

Raf kinase inhibitor protein (RKIP) dimer formation controls its target switch from Raf1 to G protein-coupled receptor kinase (GRK) 2.

Detection of anti-β1-AR autoantibodies in heart failure by a cell-based competition ELISA.

β-Adrenergic receptor stimulation causes cardiac hypertrophy via a Gβγ/Erk-dependent pathway.

Sequential inter- and intrasubunit rearrangements during activation of dimeric metabotropic glutamate receptor 1.

Administration of the cyclic peptide COR-1 in humans (phase I study): ex vivo measurements of anti-β1-adrenergic receptor antibody neutralization and of immune parameters.

Single-molecule analysis of fluorescently labeled G-protein-coupled receptors reveals complexes with distinct dynamics and organization.

Cell biology: Receptor signals come in waves.

Interference with ERK(Thr188) phosphorylation impairs pathological but not physiological cardiac hypertrophy.

Time-resolved fluorescence ligand binding for G protein-coupled receptors.

Gene amplification and functional diversification of melanocortin 4 receptor at an extremely polymorphic locus controlling sexual maturation in the platyfish.

Arrestin interactions with G protein-coupled receptors.

Crosstalk between sentinel and helper macrophages permits neutrophil migration into infected uroepithelium.

G protein-coupled receptor oligomerization revisited: functional and pharmacological perspectives.

Kinetics and mechanism of G protein-coupled receptor activation.

Constitutive activation of PKA catalytic subunit in adrenal Cushing's syndrome.

Role of membrane microdomains in compartmentation of cAMP signaling.

β-Myosin Heavy Chain Variant Val606Met Causes Very Mild Hypertrophic Cardiomyopathy in Mice, but Exacerbates HCM Phenotypes in Mice Carrying Other HCM Mutations.

Spatial and Temporal Aspects of Signaling by G-Protein-Coupled Receptors.

The ins and outs of adrenergic signaling.

A universal bioluminescence resonance energy transfer sensor design enables high-sensitivity screening of GPCR activation dynamics.

Stepwise activation of a class C GPCR begins with millisecond dimer rearrangement.