Thomas Gaj

Highly selective cyclic peptide ligands for NeutrAvidin and avidin identified by phage display.

The AviD-tag, a NeutrAvidin/avidin specific peptide affinity tag for the immobilization and purification of recombinant proteins.

Tethering small molecules to a phage display library: discovery of a selective bivalent inhibitor of protein kinase A.

Directed evolution of recombinase specificity by split gene reassembly.

Directed evolution of an enhanced and highly efficient FokI cleavage domain for zinc finger nucleases.

Structure-guided reprogramming of serine recombinase DNA sequence specificity.

Targeted plasmid integration into the human genome by an engineered zinc-finger recombinase.

Targeted gene knockout by direct delivery of zinc-finger nuclease proteins.

Chimeric TALE recombinases with programmable DNA sequence specificity.

A comprehensive approach to zinc-finger recombinase customization enables genomic targeting in human cells.

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering.

Expanding the scope of site-specific recombinases for genetic and metabolic engineering.

Regulation of endogenous human gene expression by ligand-inducible TALE transcription factors.

Expanding the zinc-finger recombinase repertoire: directed evolution and mutational analysis of serine recombinase specificity determinants.

Cell-penetrating peptide-mediated delivery of TALEN proteins via bioconjugation for genome engineering.

Enhancing the specificity of recombinase-mediated genome engineering through dimer interface redesign.

Synthetic zinc finger proteins: the advent of targeted gene regulation and genome modification technologies.

Protein delivery using Cys2-His2 zinc-finger domains.

Site-selective labeling of a lysine residue in human serum albumin.

Genome engineering with custom recombinases.

Improved cell-penetrating zinc-finger nuclease proteins for precision genome engineering.