Takuya Nishigaki

A caged sperm-activating peptide that has a photocleavable protecting group on the backbone amide.

A sea urchin egg jelly peptide induces a cGMP-mediated decrease in sperm intracellular Ca(2+) before its increase.

Measuring ion fluxes in sperm.

Sperm-activating peptide induces asymmetric flagellar bending in sea urchin sperm.

Calcium channels and Ca2+ fluctuations in sperm physiology.

Real-time analysis of the role of Ca(2+) in flagellar movement and motility in single sea urchin sperm.

Maitotoxin potently promotes Ca2+ influx in mouse spermatogenic cells and sperm, and induces the acrosome reaction.

Sperm channel diversity and functional multiplicity.

Stroboscopic illumination using light-emitting diodes reduces phototoxicity in fluorescence cell imaging.

Rho, Rho-kinase, and the actin cytoskeleton regulate the Na+ -H+ exchanger in sea urchin eggs.

Altering the speract-induced ion permeability changes that generate flagellar Ca2+ spikes regulates their kinetics and sea urchin sperm motility.

Rho-kinase (ROCK) in sea urchin sperm: its role in regulating the intracellular pH during the acrosome reaction.

Sperm-activating peptides in the regulation of ion fluxes, signal transduction and motility.

Tuning sperm chemotaxis by calcium burst timing.

The opening of maitotoxin-sensitive calcium channels induces the acrosome reaction in human spermatozoa: differences from the zona pellucida.

Tuning sperm chemotaxis.

Calcium channels in the development, maturation, and function of spermatozoa.

A caged progesterone analog alters intracellular Ca2+ and flagellar bending in human sperm.

Intracellular pH in sperm physiology.

Zn(2+) induces hyperpolarization by activation of a K(+) channel and increases intracellular Ca(2+) and pH in sea urchin spermatozoa.

FRET analysis using sperm-activating peptides tagged with fluorescent proteins reveals that ligand-binding sites exist as clusters.

Cd(2+) sensitivity and permeability of a low voltage-activated Ca(2+) channel with CatSper-like selectivity filter.