miniaturized-sample-preparation-for-transmission-electron-microscopy

Miniaturized Sample Preparation for Transmission Electron Microscopy

Due to recent technological progress, cryo-electron microscopy (cryo-EM) is rapidly becoming a standard method for the structural analysis of protein ...

University of Basel

We present a sample preparation method for cryo-electron microscopy (cryo-EM) that requires only 3-20nL of sample to prepare a cryo-EM grid, depending on the protocol used. The sample is applied and spread on the grid by a microcapillary. The procedure does not involve any blotting steps, and real-time monitoring allows the water film thickness to be assessed and decreased to an optimum value prior to vitrification. We demonstrate that the method is suitable for high-resolution cryo-EM and will enable alternative electron microscopy approaches, such as single-cell visual proteomics.

Stefan Albiez

Center for Cellular Imaging and NanoAnalytics (C-CINA),
Biozentrum,
Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum

University of Basel

Miniaturized Sample Preparation for Transmission Electron Microscopy

Stefan Albiez

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Center for Cellular Imaging and NanoAnalytics (C-CINA),Biozentrum,Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum

University of Basel

Miniaturized Sample Preparation for Transmission Electron Microscopy

Center for Cellular Imaging and NanoAnalytics (C-CINA),
Biozentrum,
Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum