the-mub40-peptide-for-use-detecting-neutrophil-mediated-inflammation

The MUB<sub>40</sub> Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

Here, we provide a protocol involving the use of MUB40, a synthesized peptide with the ability to bind glycosylated lactoferrin stored at high ...

Institut Pasteur

Université Bordeaux Segalen

Neutrophils represent the most abundant immune cells recruited to inflamed tissues. A lack of dedicated tools has hampered their detection and study. We show that a synthesized peptide, MUB, binds to lactoferrin, the most abundant protein stored in neutrophil-specific and tertiary granules. Lactoferrin is specifically produced by neutrophils among other leukocytes, making MUB a specific neutrophil marker. Naive mammalian neutrophils (human, guinea pig, mouse, rabbit) were labeled by fluorescent MUB conjugates (-Cy5, Dylight405). A peptidase-resistant retro-inverso MUB (RI-MUB) was synthesized and its lactoferrin-binding property validated. Neutrophil lactoferrin secretion during in vitro Shigella infection was assessed with RI-MUB-Cy5 using live cell microscopy. Systemically administered RI-MUB-Cy5 accumulated at sites of inflammation in a mouse arthritis inflammation model in vivo and showed usefulness as a potential tool for inflammation detection using non-invasive imaging. Improving neutrophil detection with the universal and specific MUB marker will aid the study of broad ranges of inflammatory diseases.

Louise Injarabian

Unité de Pathogénie Microbienne Moléculaire,
INSERM Unité 1202

Institut Pasteur

The MUB₄₀ Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

Louise Injarabian

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Unité de Pathogénie Microbienne Moléculaire,INSERM Unité 1202

Institut Pasteur

The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

Unité de Pathogénie Microbienne Moléculaire,
INSERM Unité 1202

The MUB₄₀ Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events