David Beebe

The function of FGF signaling in the lens placode.

Syndecan-1 in breast cancer stroma fibroblasts regulates extracellular matrix fiber organization and carcinoma cell motility.

Rapid prototyping of arrayed microfluidic systems in polystyrene for cell-based assays.

1-((3S,4S)-4-amino-1-(4-substituted-1,3,5-triazin-2-yl) pyrrolidin-3-yl)-5,5-difluoropiperidin-2-one inhibitors of DPP-4 for the treatment of type 2 diabetes.

Vitreoretinal influences on lens function and cataract.

One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST).

The tumor suppressor gene Trp53 protects the mouse lens against posterior subcapsular cataracts and the BMP receptor Acvr1 acts as a tumor suppressor in the lens.

Hax1 regulates neutrophil adhesion and motility through RhoA.

Pipette-friendly laminar flow patterning for cell-based assays.

The mechanism of lens placode formation: a case of matrix-mediated morphogenesis.

Micro- and nanoengineering for stem cell biology: the promise with a caution.

Automation of three-dimensional cell culture in arrayed microfluidic devices.

Racial differences in ocular oxidative metabolism: implications for ocular disease.

Purification of cell subpopulations via immiscible filtration assisted by surface tension (IFAST).

Anesthetic complications including two cases of postoperative respiratory depression in living liver donor surgery.

Quantification of kinase activity in cell lysates via photopatterned macroporous poly(ethylene glycol) hydrogel arrays in microfluidic channels.

Hedgehog signaling in myofibroblasts directly promotes prostate tumor cell growth.

Microscale functional cytomics for studying hematologic cancers.

Engineers are from PDMS-land, Biologists are from Polystyrenia.

Orally-effective, long-acting sorbitol dehydrogenase inhibitors: synthesis, structure-activity relationships, and in vivo evaluations of novel heterocycle-substituted piperazino-pyrimidines.

The effect of insufflation pressure on CO(2) pneumoperitoneum and embolism in piglets.

Control and applications of immiscible liquids in microchannels.

Comparison of static and dynamic medium environments for culturing of pre-implantation mouse embryos.

SAR and species/stereo-selective metabolism of the sorbitol dehydrogenase inhibitor, CP-470,711.

Physics and applications of microfluidics in biology.

Bone morphogenetic protein signaling and the initiation of lens fiber cell differentiation.

Principles of surface-directed liquid flow in microfluidic channels.

A sorbitol dehydrogenase inhibitor of exceptional in vivo potency with a long duration of action: 1-(R)-[4-[4-(4,6-dimethyl[1,3,5]triazin-2-yl)- 2R,6S-dimethylpiperazin-1-yl]pyrimidin-2- yl]ethanol.

Characterization of a silicon-based shear-force sensor on human subjects.

Evaluation of fibroblast growth factor signaling during lens fiber cell differentiation.

A highly selective, non-hydantoin, non-carboxylic acid inhibitor of aldose reductase with potent oral activity in diabetic rat models: 6-(5-chloro-3-methylbenzofuran- 2-sulfonyl)-2-H-pyridazin-3-one.

Vascular endothelial growth factor expression and signaling in the lens.

Design and synthesis of a novel family of triazine-based inhibitors of sorbitol dehydrogenase with oral activity: 1-[4-[3R,5S-dimethyl-4-(4-methyl-[1,3,5]triazin-2-yl)-piperazin-1-yl]-[1,3,5]triazin-2-yl]-(R) ethanol.

X-ray crystallographic and kinetic studies of human sorbitol dehydrogenase.

Organic and biomimetic designs for microfluidic systems.

Importance of vitreous liquefaction in age-related cataract.

Flow control with hydrogels.

Developments in in vitro technologies for swine embryo production.

Microfluidic removal of cumulus cells from Mammalian zygotes.

Mammalian embryo culture in a microfluidic device.

Microenvironment design considerations for cellular scale studies.

The expression pattern of opticin during chicken embryogenesis.

Microfluidic valve with cored glass microneedle for microinjection.

In situ fabricated porous filters for microsystems.

A passive pumping method for microfluidic devices.

An evaporation-based microfluidic sample concentration method.

A hydrogel readout for autonomous detection of ions in microchannels.

Ultra rapid prototyping of microfluidic systems using liquid phase photopolymerization.

Embryonic development in the mouse is enhanced via microchannel culture.

Microfluidic tectonics platform: A colorimetric, disposable botulinum toxin enzyme-linked immunosorbent assay system.

An externally driven magnetic microstirrer.

Expression and regulation of alpha-, beta-, and gamma-crystallins in mammalian lens epithelial cells.

Contributions by members of the TGFbeta superfamily to lens development.

Hydrodynamic microfabrication via"on the fly" photopolymerization of microscale fibers and tubes.

Magnetically-driven biomimetic micro pumping using vortices.

Vitrectomy surgery increases oxygen exposure to the lens: a possible mechanism for nuclear cataract formation.

Signaling through FGF receptor-2 is required for lens cell survival and for withdrawal from the cell cycle during lens fiber cell differentiation.

Lead exposure and cataract risk in men.

Continuous fabrication of biocatalyst immobilized microparticles using photopolymerization and immiscible liquids in microfluidic systems.

Patients want an anesthesiologist to plan and be in charge of their anesthesia.

Development of a disposable infusion system for the delivery of protein therapeutics.

Diffusion dependent cell behavior in microenvironments.

A novel series of non-carboxylic acid, non-hydantoin inhibitors of aldose reductase with potent oral activity in diabetic rat models: 6-(5-chloro-3-methylbenzofuran-2-sulfonyl)-2H-pyridazin-3-one and congeners.

Controlled microfluidic interfaces.

Reduction of polyspermic penetration using biomimetic microfluidic technology during in vitro fertilization.

Application of sexed semen technology to in vitro embryo production in cattle.

Characterization of a membrane-based gradient generator for use in cell-signaling studies.

An oral tactile interface for blind navigation.

Oxygen distribution in the rabbit eye and oxygen consumption by the lens.

Steady flow generation in microcirculatory systems.

Capillary inserts in microcirculatory systems.

A method for dynamic system characterization using hydraulic series resistance.

Mammary stem and progenitor cells: tumour precursors?

Lower intraocular oxygen tension in diabetic patients: possible contribution to decreased incidence of nuclear sclerotic cataract.

alpha-Smooth muscle actin is constitutively expressed in the lens epithelial cells of several species.

Dissolvable membranes as sensing elements for microfluidics based biological/chemical sensors.

Adaptive liquid microlenses activated by stimuli-responsive hydrogels.

Do-it-yourself microelectrophoresis chips with integrated sample recovery.

Public access success at PubMed.

Polarity effect in electrovibration for tactile display.

PDMS absorption of small molecules and consequences in microfluidic applications.

PDMS bonding by means of a portable, low-cost corona system.

Hydrogel-based reconfigurable components for microfluidic devices.

Hybrid polymer/thin film impedance system for label free monitoring of cells.

Identification and expression of Hop, an atypical homeobox gene expressed late in lens fiber cell terminal differentiation.

Autonomously-triggered microfluidic cooling using thermo-responsive hydrogels.

High-throughput microfluidics: improved sample treatment and washing over standard wells.

A plate reader-compatible microchannel array for cell biology assays.

Comment on: the Stokes-Einstein equation and the physiological effects of vitreous surgery.

Spatiotemporal micropatterning of cells on arbitrary substrates.

Lectin-modified microchannels for mammalian cell capture and purification.

Understanding microchannel culture: parameters involved in soluble factor signaling.

Microfluidic based platform for characterization of protein interactions in hydrogel nanoenvironments.

Intracellular mediators of transforming growth factor beta superfamily signaling localize to endosomes in chicken embryo and mouse lenses in vivo.

Protocol for the fabrication of enzymatically crosslinked gelatin microchannels for microfluidic cell culture.

Anaesthetic management of patients with congenital heart disease presenting for non-cardiac surgery.

Maintaining transparency: a review of the developmental physiology and pathophysiology of two avascular tissues.

Microfluidic logic gates and timers.

Flow rate analysis of a surface tension driven passive micropump.

Microscale bioengineering inspired by nature: from widgets to cell biology.

Age-dependent control of lens growth by hypoxia.

Automated cell culture in high density tubeless microfluidic device arrays.

Fibroblast growth factor receptor signaling is essential for lens fiber cell differentiation.

Myopia and axial length contribute to vitreous liquefaction and nuclear cataract.

An adaptable hydrogel array format for 3-dimensional cell culture and analysis.

Managing evaporation for more robust microscale assays. Part 1. Volume loss in high throughput assays.

Managing evaporation for more robust microscale assays. Part 2. Characterization of convection and diffusion for cell biology.

Functions of the type 1 BMP receptor Acvr1 (Alk2) in lens development: cell proliferation, terminal differentiation, and survival.

HIF-1: an age-dependent regulator of lens cell proliferation.

Cited2 is required for the proper formation of the hyaloid vasculature and for lens morphogenesis.

Microfluidics meet cell biology: bridging the gap by validation and application of microscale techniques for cell biological assays.

Quantification of small cell numbers with a microchannel device.

The function of VEGF-A in lens development: formation of the hyaloid capillary network and protection against transient nuclear cataracts.

A platform for assessing chemotactic migration within a spatiotemporally defined 3D microenvironment.

Bead-based microfluidic toxin sensor integrating evaporative signal amplification.

Biomimetic soft multifunctional miniature aquabots.

Infection on a chip: a microscale platform for simple and sensitive cell-based virus assays.

Failure rates and survival analysis of maxillary expansion appliances: a 24-year retrospective analysis.

Cell-free protein expression in a microchannel array with passive pumping.

Self-assembled peptide monolayers as a toxin sensing mechanism within arrayed microchannels.

Piperidinyl-2-phenethylamino inhibitors of DPP-IV for the treatment of type 2 diabetes.

The gel state of the vitreous and ascorbate-dependent oxygen consumption: relationship to the etiology of nuclear cataracts.

FGF-regulated BMP signaling is required for eyelid closure and to specify conjunctival epithelial cell fate.

Dosage effects of cohesin regulatory factor PDS5 on mammalian development: implications for cohesinopathies.

An accessible micro-capillary electrophoresis device using surface-tension-driven flow.

Pyridones as glucokinase activators: identification of a unique metabolic liability of the 4-sulfonyl-2-pyridone heterocycle.

Selective and tunable gradient device for cell culture and chemotaxis study.

Control of 3-dimensional collagen matrix polymerization for reproducible human mammary fibroblast cell culture in microfluidic devices.

Loss of NF-kappaB control and repression of Prdx6 gene transcription by reactive oxygen species-driven SMAD3-mediated transforming growth factor beta signaling.

Biological implications of polydimethylsiloxane-based microfluidic cell culture.

The type I BMP receptors, Bmpr1a and Acvr1, activate multiple signaling pathways to regulate lens formation.

Cellular observations enabled by microculture: paracrine signaling and population demographics.

Introducing a new Method section for Integrative Biology.

Integrating biology and technology.

The development of integrative biology: bridging the gap--a view from the Scientific Editors. [An interview with David Beebe and Mary Helen Barcellos-Hoff by Kathleen Too].

From the cellular perspective: exploring differences in the cellular baseline in macroscale and microfluidic cultures.

Fundamentals of microfluidic cell culture in controlled microenvironments.

The tumor suppressor merlin is required for cell cycle exit, terminal differentiation, and cell polarity in the developing murine lens.

Automated high-throughput microchannel assays for cell biology: Operational optimization and characterization.

Counterpoint: The lens fluid circulation model--a critical appraisal.

3D microchannel co-culture: method and biological validation.

A Cell Programmable Assay (CPA) chip.

Cell culture models in microfluidic systems.

Synaptotagmin II peptide-bead conjugate for botulinum toxin enrichment and detection in microchannels.

Expanding the available assays: adapting and validating In-Cell Westerns in microfluidic devices for cell-based assays.

Visualizing lens epithelial cell proliferation in whole lenses.

Ischemic diabetic retinopathy may protect against nuclear sclerotic cataract.

Oxygen distribution in the human eye: relevance to the etiology of open-angle glaucoma after vitrectomy.

Oxidative damage and the prevention of age-related cataracts.

A microfluidic cell concentrator.

Comparison of two probe designs for determining intraocular oxygen distribution.

An arrayed high-content chemotaxis assay for patient diagnosis.

Introduction to the mechanisms of directed cell migration themed issue.

Development of macroporous poly(ethylene glycol) hydrogel arrays within microfluidic channels.

Microfluidic cell culture and its application in high-throughput drug screening: cardiotoxicity assay for hERG channels.

Transition to invasion in breast cancer: a microfluidic in vitro model enables examination of spatial and temporal effects.