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University of Zaragoza-IACS

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Ruth Soler-Agesta¹, Joaquín Marco-Brualla¹, Patricio Fernández-Silva^1,2, Pilar Mozas^1,3, Alberto Anel¹, Raquel Moreno Loshuertos^1,2

¹Department of Biochemistry, University of Zaragoza, ²Institute for Biocomputation and Physics of Complex Systems, University of Zaragoza, ³Sequencing and Functional Genomics Unit, University of Zaragoza-IACS

This protocol describes a technique for cybrid generation from suspension-growing cancer cells as a tool to study the role of mitochondria in the tumorigenic process.