Studying Muscle Transcriptional Dynamics at Single-molecule Scales in DrosophilaEmma Leroux 1, Nourhene Ammar 1, Savannah Moinet 1, Thierry Pecot 2, Hadi Boukhatmi 1
1Institut de Génétique et Développement de Rennes (IGDR), UMR6290 CNRS - Université de Rennes, 2BIOSIT, UAR 3480 US 018, Université de Rennes
Drosophila is a well-established model for studying key molecules that regulate myogenesis. However, current methods are insufficient to determine mRNA transcriptional dynamics and spatial distribution within syncytia. To address this limitation, we optimized an RNA fluorescence in situ hybridization method allowing the detection and quantification of mRNAs at a single-molecule scale.
