Hepatic Glucose Production, Ureagenesis, and Lipolysis Quantified using the Perfused Mouse Liver ModelMarie Winther-Sørensen 1,2,3, Ida Marie Kemp 1,3, Hanne Cathrine Bisgaard 4, Jens Juul Holst 1,5, Nicolai J. Wewer Albrechtsen 2,3
1Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2NNF Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 3Department for Clinical Biochemistry, Copenhagen University Hospital - Bispebjerg and Frederiksberg, 4Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 5NNF Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen
Here, we present a robust method for in situ perfusion of the mouse liver to study the acute and direct regulation of liver metabolism without disturbing the hepatic architecture but in the absence of extra-hepatic factors.
