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The Broad Institute of MIT and Harvard

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)

Wei Jiang¹, Holly Soutter¹

¹Center for the Development of Therapeutics, The Broad Institute of MIT and Harvard

Here we describe protocols for the biophysical characterization of ternary complex formation induced by proteolysis targeting chimeras (PROTACS) that involve the ubiquitin ligases Von Hippel-Lindau E3 ligase (VHL) and Cereblon (CRBN). Biophysical methods illustrated herein include surface plasmon resonance (SPR), biolayer interferometry (BLI), and isothermal titration calorimetry (ITC).