Cell-Free DNA Extraction of Vitreous and Aqueous Humor Specimens for Diagnosis and Monitoring of Vitreoretinal LymphomaNoah A. Brown 1, Rajesh C. Rao 1,2,3,4,5,6,7,8, Bryan L. Betz 1
1Department of Pathology, University of Michigan, Ann Arbor, 2Department of Ophthalmology and Visual Sciences, Kellogg Eye Center, University of Michigan, Ann Arbor, 3Department of Human Genetics, University of Michigan, Ann Arbor, 4Rogel Cancer Center, University of Michigan, Ann Arbor, 5Center of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, 6Center for RNA Biomedicine, University of Michigan, Ann Arbor, 7A. Alfred Taubman Medical Research Institute, University of Michigan, Ann Arbor, 8Section of Ophthalmology, Surgery Service, Veterans Administration Ann Arbor Healthcare System
A procedure to extract cell-free DNA from vitreous and aqueous humor to perform molecular studies for diagnosing vitreoretinal lymphoma is established here. The method offers the ability to concurrently extract DNA from the cellular component of the sample or to reserve it for ancillary testing.
