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Singapore-MIT Alliance for Research and Technology Antimicrobial Resistance Interdisciplinary Research Group

1 ARTICLES PUBLISHED IN JoVE

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Genetics

AQRNA-seq for Quantifying Small RNAs
Ruixi Chen 1, Daniel Yim 2, Peter C. Dedon 1,2
1Department of Biological Engineering, Massachusetts Institute of Technology, 2Singapore-MIT Alliance for Research and Technology Antimicrobial Resistance Interdisciplinary Research Group

Absolute quantification RNA sequencing (AQRNA-seq) is a technology developed to quantify the landscape of all small RNAs in biological mixtures. Here, both the library preparation and data processing steps of AQRNA-seq are demonstrated, quantifying changes in the transfer RNA (tRNA) pool in Mycobacterium bovis BCG during starvation-induced dormancy.

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