Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative ScaleMichael J. Stephenson 1, James Reed 1, Bastiaan Brouwer 1, Anne Osbourn 1
1John Innes Centre
Transient heterologous expression of biosynthetic enzymes in Nicotiana benthamiana leaves can divert endogenous supplies of 2,3-oxidosqualene towards the production of new high-value triterpene products. Herein is described a detailed protocol for rapid (5 days) preparative-scale production of triterpenes and analogs utilizing this powerful plant-based platform.
