Comparative Study of Basement-Membrane Matrices for Human Stem Cell Maintenance and Intestinal Organoid GenerationJanny Pineiro-Llanes 1, Lais da Silva 1, John Huang 2, Rodrigo Cristofoletti 1
1Center for Pharmacometrics & Systems Pharmacology, Department of Pharmaceutics, University of Florida, 2TheWell Bioscience, Inc
Organoids have become valuable tools for disease modeling. The extracellular matrix (ECM) guides cell fate during organoid generation, and using a system that resembles the native tissue can improve model accuracy. This study compares the generation of induced pluripotent stem cells-derived human intestinal organoids in animal-derived ECM and xeno-free hydrogels.
