Three-Dimensional Cell Culture Models to Investigate the Epithelial Barrier in Eosinophilic Esophagitis Tanay Kaymak 1, Jan Hendrik Niess 1,2
1Department of Biomedicine, University of Basel, 2Department of Gastroenterology and Hepatology, University Digestive Healthcare Center, Clarunis
Here, a protocol for the culture of human esophageal organoids and air-liquid interface culture is provided. Esophageal organoids' air-liquid interface culture can be used to study the impact of cytokines on the esophageal epithelial barrier.