Surgical Perfusion and Isolation of the Porcine Pancreas for Islet IsolationSophia H. Roberts 1,2, Louai Alrata 1,2, Dahlia Abdulsattar 1,2, Karan Joseph 1,2, Wahid Abu-Amer 1,2, Mohamed Zaghloul 1,2, Batool Arif 1,2, Tarek Alhamad 3, Maria S. Remedi 4, Yiing Lin 5, Mohamed A. Zayed 1,2,6,7
1Department of Surgery, Section of Vascular Surgery, Washington University School of Medicine, 2CardioVascular Research Innovation in Surgery and Engineering Center, Washington University School of Medicine, 3Department of Medicine, Division of Nephrology, Washington University School of Medicine, 4Department of Medicine, Division of Endocrinology, Washington University School of Medicine, 5Department of Surgery, Section of Transplant Surgery, Washington University School of Medicine, 6Division of Molecular Cell Biology, Washington University School of Medicine, 7Department of Biomedical Engineering, Washington University McKelvey School of Engineering
This protocol provides a step-by-step guide for the procurement of a porcine pancreas for islet isolation and purification.
