Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated SystemSayaka Fuseya 1, Ayaka Ono 1, Hiromi Ootani 1, Saho Mizukado 1, Tomomi Obayashi 2, Nana Tanaka 2, Hiroko Shimazaki 1,2, Kenji Kajiyama 2, Moe Ashitomi 2, Shiori Yasuda 2, Takenori Miyabe 2, Kazuhiro Nakamura 2, Osamu Segawa 2, Kazumi Sawakami 2, Atsushi Kuno 1
1Molecular & Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, 2Precision System Science Co., Ltd.
A system for the automated and rapid analysis of glycans on proteins was previously developed. This article presents the protocol for "glyco-qualitative analysis," optimized for a broad spectrum of users, such as those engaged in analyzing glycan structures in biopharmaceuticals and other glycoconjugate materials.
