Elucidating β-1,3-Glucanase and Peroxidase Physicochemical Properties of Wheat Cell Wall Defense Mechanism Against Diuraphis noxia InfestationSiphephelo N.N. Zondo 1, Lintle Mohase 2, Vicki Tolmay 3, Mpho S. Mafa 1
1Carbohydrates and Enzymology Laboratory (CHEM-LAB), Department of Plant Sciences, University of the Free State, 2Faculty of Natural and Agricultural Sciences, Department of Plant Science, Botany, Plant Biochemistry and Physiology, University of the Free State, 3Agricultural Research Council - Small Grain Institute
The present protocol describes procedures used to study and characterize cell wall-related enzymes, mainly β-1,3-glucanase and peroxidase, in wheat plants. Their activity levels increase during wheat-RWA interaction and are involved in the plant defense response through cell wall reinforcement, which deters aphid feeding.
