Split-Luciferase Reassembly Assay to Measure Endoplasmic Reticulum-Mitochondria Contacts in Live CellsConnie Chen *1, Katherine A. Rafael *1, Ginam Cho 1, Youngshin Lim 1,2
1Department of Pathology, Cedars-Sinai Medical Center, 2Department of Biomedical Science Education, College of Medicine, Charles R. Drew University of Medicine and Science
We have established a split-luciferase reassembly assay to monitor the endoplasmic reticulum-mitochondria contacts in live cells. Using this assay, we describe a protocol to quantitatively measure the level of these inter-organelle couplings in HEK293T cells, under the condition of chemical treatment.
