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Kyoto Prefectural University of Medicine

4 ARTICLES PUBLISHED IN JoVE

Behavior

Clinical-oriented Three-dimensional Gait Analysis Method for Evaluating Gait Disorder

Masahiko Mukaino¹, Kei Ohtsuka², Hiroki Tanikawa², Fumihiro Matsuda², Junya Yamada³, Norihide Itoh⁴, Eiichi Saitoh¹

¹Department of Rehabilitation Medicine, School of Medicine, Fujita Health University, ²Faculty of Rehabilitation, School of Health Science, Fujita Health University, ³Department of Rehabilitation, Fujita Health University Hospital, ⁴Department of Advanced Rehabilitation Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

In this study, a clinician-friendly three-dimensional gait analysis method, which was designed to be performed in the rehabilitation clinic, is presented. The method consists of a simplified measurement method and intuitive figures to facilitate clinicians' understanding of the results.

Medicine

Designing CAD/CAM Surgical Guides for Maxillary Reconstruction Using an In-house Approach

Toshiaki Numajiri¹, Daiki Morita², Hiroko Nakamura³, Ryo Yamochi¹, Shoko Tsujiko⁴, Yoshihiro Sowa¹

¹Department of Plastic and Reconstructive Surgery, Kyoto Prefectural University of Medicine, ²Department of Plastic and Reconstructive Surgery, Showa University Fujigaoka Hospital, ³Department of Plastic and Reconstructive Surgery, Fukuchiyama City Hospital, ⁴Department of Plastic and Reconstructive Surgery, Saiseikai Shiga Hospital

Methods for designing a computer-aided design/computer-aided manufacturing (CAD/CAM) surgical guide are shown. Cutting planes are separated, united, and thickened to easily visualize the necessary bone transfer. These designs can be three-dimensional printed and checked for accuracy.

Bioengineering

A New Ex Vivo Model for the Evaluation of Endoscopic Submucosal Injection Material Performance

Ryohei Hirose^1,2, Tomo Daidoji², Yuji Naito¹, Osamu Dohi¹, Naohisa Yoshida¹, Hiroaki Yasuda¹, Hideyuki Konishi¹, Takaaki Nakaya², Yoshito Itoh¹

¹Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, ²Department of Infectious Diseases, Graduate School of Medical Science, Kyoto Prefectural University of Medicine

We developed a new ex vivo model that applies constant tension to the porcine gastric specimen. This development made it possible to evaluate the performance (the height and duration of the submucosal elevation) of various SIMs accurately. The detailed setup methodology of this new model is explained.

Developmental Biology

In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells

Chuang-Yu Lin¹, Michiko Yoshida^1,2, Li-Tzu Li³, Megumu K. Saito¹

¹Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, ²Department of Pediatrics, Kyoto Prefectural University of Medicine, ³Graduate Institute of Clinical Medicine, Taipei Medical University

Here we provide a protocol to generate in vitro NMJs from human induced pluripotent stem cells (iPSCs). This method can induce NMJs with mature morphology and function in 1 month in a single well. The resulting NMJs could potentially be used to model related diseases, to study pathological mechanisms or to screen drug compounds for therapy.