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University of Salerno

1 ARTICLES PUBLISHED IN JoVE

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Biology

Production of Cardiac Extracellular Matrix from Adult Human Fibroblasts for Culture Dish Coating
Daria Nurzynska 1, Anna Maria Sacco 2, Valentino Servodio 2, Veronica Romano 2, Immacolata Belviso 2, Clotilde Castaldo 2, Franca Di Meglio 2
1Department of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 2Department of Public Health, University of Naples Federico II

Fibroblasts isolated from the adult human heart were cultured to confluence on gelatin-coated dishes to produce the myocardium-specific extracellular matrix. After decellularization, this substrate can be used for the culture and study of other cardiac cells and cell-matrix interactions.

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