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University of Louisville School of Medicine

5 ARTICLES PUBLISHED IN JoVE

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Biology

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
Nazarul Hasan 1, David Humphrey 1, Krista Riggs 1, Chuan Hu 1
1Department of Biochemistry and Molecular Biology, University of Louisville School of Medicine

We have developed a cell fusion assay that quantifies SNARE-mediated membrane fusion events by activated expression of β-galactosidase.

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Medicine

Controlled Cervical Laceration Injury in Mice
Yi Ping Zhang 1, Melissa J. Walker 2, Lisa B. E. Shields 1, Xiaofei Wang 2, Chandler L. Walker 2, Xiao-Ming Xu 2, Christopher B. Shields 1
1Norton Neuroscience Institute, Norton Healthcare, 2Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Medical Neuroscience Graduate Program, and Department of Anatomy and Cell Biology, Indiana University School of Medicine

A novel technique to create a reproducible in vivo model of cervical spinal cord laceration injury in the mouse is described. This technique is based on spine stabilization by fixation of the cervical facets and laceration of the spinal cord using an oscillating blade with an accuracy of ±0.01 mm.

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Medicine

A Novel Vertebral Stabilization Method for Producing Contusive Spinal Cord Injury
Melissa J. Walker *1,2, Chandler L. Walker *1,3, Y. Ping Zhang 4, Lisa B. E. Shields 4, Christopher B. Shields 4, Xiao-Ming Xu 1,3
1Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Indiana University School of Medicine, 2Medical Neuroscience Graduate Program, Indiana University School of Medicine, 3Department of Anatomy and Cell Biology, Indiana University School of Medicine, 4Norton Neuroscience Institute, Norton Healthcare

Vertebral stabilization is necessary for minimizing variability, and for producing consistent experimental spinal cord injuries.  Using a customized stabilizing apparatus in conjunction with the NYU/MASCIS impactor device, we have demonstrated here the proper equipment and procedure for generating reproducible hemi-contusive cervical (C5) spinal cord injuries in adult rats.

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Medicine

A Tissue Displacement-based Contusive Spinal Cord Injury Model in Mice
Xiangbing Wu *1, Yi Ping Zhang *2, Wenrui Qu 1, Lisa B. E. Shields 2, Christopher B. Shields 2,3, Xiao-Ming Xu 1
1Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Indiana University School of Medicine, 2Norton Neuroscience Institute, Norton Healthcare, 3Department of Anatomical Sciences and Neurobiology, University of Louisville

We introduce a tissue displacement-based contusive spinal cord injury model that can produce a consistent contusive spinal cord injury in adult mice.

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Neuroscience

An In Vivo Duo-color Method for Imaging Vascular Dynamics Following Contusive Spinal Cord Injury
Chen Chen 1,2, Yi Ping Zhang 3, Yan Sun 1,4, Wenhui Xiong 1, Lisa B. E. Shields 3, Christopher B. Shields 3,5, Xiaoming Jin 1, Xiao-Ming Xu 1
1Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, and Department of Neurological Surgery, Indiana University School of Medicine, 2Program in Medical Neuroscience, Stark Neurosciences Research Institute, Indiana University School of Medicine, 3Norton Neuroscience Institute, Norton Healthcare, 4Department of Human Anatomy & Histoembryology, School of Basic Medical Sciences, Fudan University, 5Department of Neurological Surgery, University of Louisville School of Medicine

We introduce an in vivo imaging method using two different fluorescent dyes to track dynamic spinal vascular changes following a contusive spinal cord injury in adult Sprague-Dawley rats.

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