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St Vincent's Hospital

2 ARTICLES PUBLISHED IN JoVE

Medicine

Ovine Lumbar Intervertebral Disc Degeneration Model Utilizing a Lateral Retroperitoneal Drill Bit Injury

Kai-Zheong Lim^*1, Christopher D. Daly^*1,2,3, Peter Ghosh⁴, Graham Jenkin³, David Oehme⁵, Justin Cooper-White^6,7, Taryn Naidoo⁶, Tony Goldschlager^1,8

¹Department of Surgery, Monash University, ²Department of Neurosurgery, Monash University, ³The Ritchie Centre, Hudson Institute of Medical Research, ⁴Proteobioactives, Pty Ltd, ⁵Department of Neurosurgery, St Vincent's Hospital, ⁶Australian Institute for Bioengineering and Nanotechnology, University of Queensland, ⁷School of Chemical Engineering, University of Queensland, ⁸Department of Neurosurgery, Monash Health

Intervertebral disc degeneration is a significant contributor to back pain and a leading cause of disability worldwide. Numerous animal models of intervertebral disc degeneration exist. We demonstrate an ovine model of intervertebral disc degeneration, utilizing a drill bit, which achieves a consistent disc injury and reproducible level of disc degeneration.

Medicine

Using an Isolated Working Rat Heart Model to Test Donor Heart Preservation Strategies

Jeanette E. Villanueva^1,2, Ling Gao¹, Sanjay Dutta^1,2, Aoife Doyle¹, Yashutosh Joshi^1,2, Peter S. Macdonald^1,3,4

¹Cardiac Physiology and Transplantation, Victor Chang Cardiac Research Institute, ²St Vincent's Healthcare Clinical Campus, University of New South Wales, ³Heart and Lung Transplant Unit, St Vincent's Hospital, ⁴Faculty of Medicine, University of New South Wales

We describe an ex vivo isolated working rat heart protocol to test donor heart preservation strategies. This paper describes the protocol for use in static cold storage of rodent donor hearts; however, the protocol can also be used for donor hearts obtained after donation after circulatory death and brain death.