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St. Joseph's University

1 ARTICLES PUBLISHED IN JoVE

Biology

A Simple Protocol for Mapping the Plant Root System Architecture Traits

Devesh Shukla^1,2, Prabodh Kumar Trivedi^2,3, Shivendra Sahi^1,4

¹Department of Biology, Western Kentucky University, ²Plant Biotechnology Division, CSIR Central Institute of Medicinal and Aromatic Plants, ³Academy of Scientific and Innovative Research (AcSIR), ⁴Department of Biology, St. Joseph's University

We use simple laboratory tools to examine the root system architecture (RSA) of Arabidopsis and Medicago. The plantlets are grown hydroponically over mesh and spread using an art brush to reveal the RSA. Images are taken using scanning or a high-resolution camera, then analyzed with ImageJ to map traits.