ST. JOSEPH'S HOSPITAL AND MEDICAL CENTER
2 ARTICLES PUBLISHED IN JoVE
Biology
An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells
Landon J. Inge 1, Aaron J. Fowler 1, Ross M. Bremner 1
1Center for Thoracic Disease and Transplantation, Heart and Lung Institute, St. Joseph's Hospital and Medical Center
There is a discernible need for improved information on molecular drivers of Barrett’s Esophagus. Immunofluorescent staining is a useful technique for understanding the effects of cell signaling on cell morphology. We present a simple, effective protocol for the use of immunofluorescent staining to assess therapeutic treatment in Barrett’s Esophagus cells.
Immunology and Infection
Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages
Deepak K. Nayak 1, Oscar Mendez 1, Sara Bowen 2, Thalachallour Mohanakumar 1
1Norton Thoracic Institute, St. Joseph's Hospital and Medical Center, 2Flow Cytometry Core, St. Joseph's Hospital and Medical Center
This communication describes methodologies for isolation and culture of alveolar macrophages from humans and murine models for experimental purposes.
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