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James Cook University

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians

Laura A. Brannelly¹

¹School of Public Health, Tropical Medicine and Rehabilitation Sciences, James Cook University

Batrachochytrium dendrobatidis infection in amphibians can be eliminated using a reduced concentration (0.0025%) and shorter duration (five minute baths for six days) of itraconazole than is generally used. Less mortality and fewer negative side effects were observed using this modified treatment protocol.

Immunology and Infection

Using Terminal Transferase-mediated dUTP Nick End-labelling (TUNEL) and Caspase 3/7 Assays to Measure Epidermal Cell Death in Frogs with Chytridiomycosis

Laura A. Brannelly^1,2, Alexandra A. Roberts¹, Lee F. Skerratt¹, Lee Berger¹

¹One Health Research Group, College of Public Health, Medical and Veterinary Sciences, James Cook University, ²Department of Biological Sciences, University of Pittsburgh

We quantify epidermal cell death in frogs with chytridiomycosis using two methods. First, we use terminal transferase-mediated dUTP nick end-labelling (TUNEL) in situ histology to determine differences between clinically infected and uninfected animals. Second, we conduct a time series analysis of apoptosis over infection using a caspase 3/7 protein analysis.