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3 ARTICLES PUBLISHED IN JoVE

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Biology

A Method For Production of Recombinant mCD1d Protein in Insect Cells.
Archana Khurana 1, Mitchell Kronenberg 1
1Department of Developmental Immunology, La Jolla Institute for Allergy and Immunology

A Method to prepare Insect cells and infect them with baculovirus for the the purpose of production of recombinant mCD1d proteinand generating mCD1d tetramers.

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Biology

Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation
Mitchell Kronenberg 1
1Department of Developmental Immunology, La Jolla Institute for Allergy and Immunology

Natural Killer T cells (NKT) are critical determinants of the immune response to cancer, regulation of autioimmunity, clearance of infection, and the development of artheriosclerotic plaques. In this interview, Mitch Kronenberg discusses his laboratory's efforts to understand the mechanism through which NKT cells are activated by glycolipid antigens.

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Immunology and Infection

Mouse Body Temperature Measurement Using Infrared Thermometer During Passive Systemic Anaphylaxis and Food Allergy Evaluation
Yu Kawakami 1, Rachel Sielski 1, Toshiaki Kawakami 1
1Division of Cell Biology, La Jolla Institute for Allergy and Immunology

Here we present a new method to accurately measure body temperature differences in passive systemic anaphylaxis (PSA) and food allergy mouse models using an infrared thermometer. This procedure has been accurately duplicated in previous PSA results.

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