A Burrowing/Tunneling Assay for Detection of Hypoxia in Drosophila melanogaster LarvaeKaren M. Qiang 1,2, Fanli Zhou 1, Kathleen M. Beckingham 1
1Department of Biosciences, Rice University, 2Yale Medical School
The protocol describes a simple assay to identify Drosophila melanogaster larvae that are experiencing hypoxia under normal atmospheric oxygen levels. This protocol allows hypoxic larvae to be distinguished from other mutants that show overlapping phenotypes such as sluggishness or slow growth.
