Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung CancerMilica Momcilovic 1, Sean T. Bailey 2, Jason T. Lee 3, Charles Zamilpa 3, Anthony Jones 3, Gihad Abdelhady 1, James Mansfield 4, Kevin P. Francis 5, David B. Shackelford 1
1Division of Pulmonary and Critical Care Medicine, University of California Los Angeles David Geffen School of Medicine, 2University of North Carolina at Chapel Hill, 3Department of Molecular and Medical Pharmacology, University of California Los Angeles, 4Andor Technology, 5Division of Orthopaedic Surgery, University of California Los Angeles David Geffen School of Medicine
In this protocol, we describe how to utilize [18F]-2-fluoro-2-deoxy-D-glucose positron emission tomography and computed tomography (18F-FDG PET/CT) imaging to measure the tumor metabolic response to the targeted therapy MLN0128 in a Kras/Lkb1 mutant mouse model of lung cancer and coupled imaging with high resolution ex vivo autoradiography and quantitative histology.
