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University of Barcelona- Scientific and Technological Centers

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

Mireia Ferrer^*1, Lorena Martin-Jaular^*1, Maria Calvo², Hernando A. del Portillo^1,3

¹Department of poverty related diseases, Barcelona Centre for International Health Research, ²Confocal Microscopy Unit, University of Barcelona- Scientific and Technological Centers, ³Institució Catalana de Recerca i Estudis Avançats (ICREA)

We show the method for performing intravital microscopy of the spleen using GFP transgenic malaria parasites and the quantification of parasite mobility and blood flow within this organ.