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Parc scientifique de Luminy

2 ARTICLES PUBLISHED IN JoVE

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Biology

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)
Vincent Rouger 1,2,3, Nicolas Bertaux 4,5,6, Tomasz Trombik 1,2,3, Sébastien Mailfert 1,2,3, Cyrille Billaudeau 1,2,3, Didier Marguet 1,2,3, Arnauld Sergé 1,2,3
1Institut National de la Santé et de la Recherche Médicale, UMR 631, Parc scientifique de Luminy, 2Centre National de la Recherche Scientifique, UMR 6102, Parc scientifique de Luminy, 3Centre d'Immunologie de Marseille-Luminy, Aix-Marseille University, 4École Centrale Marseille, Technopôle de Château-Gombert, 5Institut Fresnel, Aix-Marseille University, 6Centre National de la Recherche Scientifique, UMR 6133, Aix-Marseille University

Multiple-Target Tracing is a homemade algorithm developed for tracking individually labeled molecules within the plasma membrane of living cells. Efficiently detecting, estimating and tracing molecules over time at high-density provide a user-friendly, comprehensive tool to investigate nanoscale membrane dynamics.

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Biochemistry

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
Sebastien Mailfert *1, Karolina Wojtowicz *2, Sophie Brustlein *1, Ewa Blaszczak 3, Nicolas Bertaux 4, Marcin Łukaszewicz 2, Didier Marguet 1, Tomasz Trombik 2
1CNRS, Inserm, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Univ, 2Faculty of Biotechnology, University of Wroclaw, 3Faculty of Biological Sciences, University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel, Aix Marseille Univ

This article aims to present a protocol on how to build a spot variation Fluorescence Correlation Spectroscopy (svFCS) microscope to measure molecular diffusion at the plasma membrane of living cells.

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