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Keck Graduate Institute of Applied Life Sciences

3 ARTICLES PUBLISHED IN JoVE

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Biology

Electrophoretic Separation of Proteins
Bulbul Chakavarti 1, Deb Chakavarti 1
1Proteomic Center, Keck Graduate Institute of Applied Life Sciences

In this video, we demonstrate a method for electrophoretic separation of proteins using poly-acrylimide gel electrophoresis (PAGE).

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Biology

Immunoblot Analysis
Sean Gallagher 1, Deb Chakavarti 2
1UVP, LLC, 2Proteomic Center, Keck Graduate Institute of Applied Life Sciences

Immunoblotting (western blotting) is a rapid and sensitive assay for the detection and characterization of proteins that works by exploiting the specificity inherent in antigen-antibody recognition. This video provides protocols for protein separation, blotting proteins onto membranes, immunoprobing, and visualization using chromogenic or chemiluminescent substrates.

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Biology

Staining Proteins in Gels
Sean Gallagher 1, Deb Chakavarti 2
1Keck Graduate Institute of Applied Life Sciences, UVP, LLC, 2Proteomic Center, Keck Graduate Institute of Applied Life Sciences

Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. Staining of proteins with Coomassie Blue, Silver Staining, SYPRO Orange, SYPRO Ruby are demonstrated in this video.

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