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Ege University

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

Alev Tascioglu Aliyev^1,2, Francesca LoBianco¹, Kimberly J. Krager¹, Nukhet Aykin-Burns¹

¹Division of Radiation Health, Pharmaceutical Sciences, University of Arkansas for Medical Sciences, ²Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ege University

This protocol describes the assessment of subcellular compartment-specific redox status within the cell. A redox-sensitive fluorescent probe allows convenient ratiometric analysis in intact cells.