Rapid, Affordable, and Uncomplicated Production of Bacterial Cell-free LysateRobert M. Cooper *1, Taishi Tonooka *1,2, Andriy Didovyk *1,3, Jeff Hasty 1,4,5
1Biocircuits Institute, University of California, San Diego, 2Present address, Kyoto Institute of Technology, 3Present address, Vertex Pharmaceuticals, 4Department of Bioengineering, University of California, San Diego, 5Molecular Biology Section, University of California, San Diego
This protocol describes a rapid and simple method to produce bacterial lysate for cell-free gene expression, using an engineered strain of Escherichia coli and requiring only standard laboratory equipment.
