A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector ConstructionMing Yang *1,2, Lijuan Cui *3, Xuesha Liu 1
1College of Life Sciences, China West Normal University, 2Sichuan Sports College, 3Pathology Department, Suining Central Hospital
Here, we present a protocol to obtain the pVAX1-PRRSV expression vector by introducing suitable restriction sites at the 3' end of the inserts. We can linearize the vector and join DNA fragments to the vector one by one through homologous recombination technology.
