Glycan Profiling of Plant Cell Wall Polymers using MicroarraysIsabel E. Moller 1,2, Filomena A. Pettolino 3, Charlie Hart 1, Edwin R. Lampugnani 1, William G.T. Willats 4, Antony Bacic 1,2
1Australian Centre of Excellence in Plant Cell Walls, School of Botany, University of Melbourne, 2Plant Cell Biology Research Centre, School of Botany, University of Melbourne, 3CSIRO Plant Industry, Black Mountain Laboratories, 4Department of Plant Biology and Biotechnology, University of Copenhagen
A technique called Comprehensive Microarray Polymer Profiling (CoMPP) for the characterisation of plant cell wall glycans is described. This method combines the specificity of monoclonal antibodies directed to defined glycan-epitopes with a miniature microarray analytical platform allowing screening of glycan occurrence in a broad range of biological contexts.
