Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell AnalysisEnJun Yang 1, Jacqueline L.Y. Chee 1, Suhanya Duraiswamy 2, Siyi Chen 3, Kristin Lees 3, Swaine L. Chen 1,3
1Infectious Diseases Group, Genome Institute of Singapore, 2Department of Chemical and Biomolecular Engineering, National University of Singapore, 3Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore
This protocol describes a simple method of isolating single, infected-bladder epithelial cells from a murine model of urinary tract infection.
